不同毒力猪肺炎支原体侵入宿主细胞的观察

doi:10.11843/j.issn.0366-6964.2014.06.017

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (6): 981-988.doi: 10.11843/j.issn.0366-6964.2014.06.017

不同毒力猪肺炎支原体侵入宿主细胞的观察

车巧林，刘蓓蓓，刘茂军，熊祺琰，邵国青^*

（江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室国家兽用生物制品工程技术研究中心，南京 210014）

收稿日期:2013-10-14 出版日期:2014-06-23 发布日期:2014-06-23
通讯作者: 邵国青，研究员，E-mail：gqshaojaas@gmail.com
作者简介:车巧林（1982-），女，山西吕梁人，助理研究员，博士，主要从事猪肺炎支原体致病机理研究，E-mail：cheqiaolin@163.com
基金资助:
国家自然科学基金（31100136）；中国博士后面上项目（2012M510175）

Invasion of Host Cells by Different Virulent Mycoplasma hyopneumoniae

CHE Qiao-lin，LIU Bei-bei，LIU Mao-jun，XIONG Qi-yan，SHAO Guo-qing^*

(Institute of Veterinary Medicine，Jiangsu Academy of Agricultural Sciences，Key Laboratory of Veterinary Biological Engineering and Technology of Ministry of Agriculture，National Center for Engineering Research of Veterinary Bio-products，Nanjing 210014 ， China)

Received:2013-10-14 Online:2014-06-23 Published:2014-06-23

摘要/Abstract

摘要：

旨在研究不同毒力猪肺炎支原体对宿主细胞的侵入以及侵入后在宿主细胞内的分布位置。首先用猪肺炎支原体野毒株XLW-1感染PK15细胞不同时间后，间接免疫荧光染色，激光扫描共聚焦显微镜对PK15细胞进行断层扫描，以观察XLW-1能否侵入细胞以及感染时间对XLW-1侵入细胞的影响。然后，在能侵入细胞的基础上，用不同毒力的猪肺炎支原体感染宿主细胞STEC、PK15、SJPL和3D4/21，观察它们对宿主细胞的侵入情况。结果表明，猪肺炎支原体野毒株XLW-1与PK15细胞作用8 h时不能侵入PK15，作用10 h以上时可以侵入PK15，并且随着作用时间的延长侵入的猪肺炎支原体也在增加。研究还表明猪肺炎支原体野毒株XLW-1、168致弱株和168疫苗株均能侵入STEC、PK15、SJPL和3D4/21。并且，STEC中3个菌株均随机分布在细胞质中；PK15中3个菌株均主要分布在靠近细胞膜和细胞两端狭长的细胞质中；SJPL中XLW-1和168疫苗株主要分布在贴近细胞膜内侧的细胞质中，168致弱株则散布在细胞质中；3D4/21中XLW-1和168致弱株主要分布在贴近细胞膜内侧的细胞质中，168疫苗株则在整个细胞质中分布较多。结果提示不同毒力的猪肺炎支原体均能侵入宿主细胞STEC、PK15、SJPL和3D4/21。

Abstract:

This experiment was conducted to study the invasion and location in host cells of different virulent Mycoplasma hyopneumoniae.The ability of wild strain XLW-1 to invade PK15 cells at different infection time interval was investigated firstly by using indirect immunofluorescence technique and laser scanning confocal microscope.Then，the invasion to swine tracheal epithelial cells (STEC)，PK15，SJPL and 3D4/21 of different virulent M.hyopneumoniae strains was observed.The results showed that 1) M.hyopneumoniae wild strains XLW-1 can intrude into PK15 cells up to 10 h post infection and no internalization was observed before 8 h; 2) The amount of intracellular M.hyopneumoniae XLW-1 was increased as infection time; 3) M.hyopneumoniae wild strainsXLW-1，168 attenuated strains and vaccine strains all could invade STEC，PK15，SJPL and 3D4/21 cells; 4) The ingested wild strainsXLW-1，168 attenuated strains and vaccine strains were randomly distributed in the cytoplasm of STEC，and mainly distributed in the cytoplasm of long and narrow two polars and cytoplasm near cytomembrane in PK15; The internalized wild strainsXLW-1 and 168 vaccine strains principally concentrated in the cytoplasm close to medial surface of SJPL cells membrane，but 168 attenuated strains randomly interspersed in the cytoplasm of SJPL cells; The incursive wild strainsXLW-1 and 168 attenuated strains principally distributed in the cytoplasm close to inboard surface of 3D4/21 cytomembrane，and 168 vaccine strains randomly spreaded in the cytoplasm of 3D4/21 cells.These results demonstated that M.hyopneumoniae of different virulence all can invade host cells STEC，PK15，SJPL and 3D4/21.

中图分类号:

S852.62

车巧林，刘蓓蓓，刘茂军，熊祺琰，邵国青. 不同毒力猪肺炎支原体侵入宿主细胞的观察[J]. 畜牧兽医学报, 2014, 45(6): 981-988.

CHE Qiao-lin，LIU Bei-bei，LIU Mao-jun，XIONG Qi-yan，SHAO Guo-qing. Invasion of Host Cells by Different Virulent Mycoplasma hyopneumoniae[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 981-988.

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不同毒力猪肺炎支原体侵入宿主细胞的观察

Invasion of Host Cells by Different Virulent Mycoplasma hyopneumoniae

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